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Catalog Number | orb257584 |
---|---|
Category | Proteins |
Description | Human IL-18 R1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # AAH69575). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 62.4 kDa |
Target | IL-18 R1 |
UniProt ID | Q13478 |
Source | Human IL-18 R1, Fc Tag (orb257584) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # AAH69575). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human IL-18 R1, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 0.409 μM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Ala 19 - Arg 329 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH69575 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL18R1,IL1RRP,CD218a,IL18Ra Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 62.4 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-18 R1 protein
Human IL-18 R1, Fc Tag (Cat. No. orb257584) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 0.409 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotin | |
95% | |
63.9 kDa | |
Biotinylated Human IL-18 R1, Fc, (orb1152438) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # AAH69575.1). |
Biotin | |
95% | |
39.3 kDa | |
Biotinylated Human IL-18 R1, His, (orb1184767) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # Q13478-1). |