Human IL-17F antibody (Biotin)

• Catalog Number: orb345116
• Category: Antibodies
• Description: Human IL-17F antibody (Biotin)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4H1629.1
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000-1:50,000, IHC: 5µg/mL, WB: 0.5 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined.
• Conjugation: Biotin
• UniProt ID: Q96PD4
• NCBI: NP_443104.1
• Storage: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-IL-17F biotin conjugated antibody, mous
• Note: For research use only
• Application notes: This purified antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of Mouse Anti-IL-17F antibody. Tissue: human colon tissue. Fixation: formalin-fixed, paraffin-embedded. Primary antibody: isotype control (top left), Mouse Anti-IL-17F antibody (right and bottom left) at 5 ug/ml.

Western Blot of Mouse Anti-IL-17F antibody. Lane 1: human full length recombinant IL-17F protein. Lane 2: mouse full length recombinant IL-17F protein. Lane 3: rat full length recombinant IL-17F protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17F antibody (orb345104) at 0.5 ug/ml for overnight at 4°C.