You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb612134 |
---|---|
Category | Proteins |
Description | Human IL-13 R alpha 2, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 39.0 kDa |
Target | IL-13 R alpha 2 |
UniProt ID | Q14627 |
Source | Human IL-13 R alpha 2, His Tag (orb612134) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IL-13, Fc Tag on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Asp 27 - Arg 343 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000631.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL13RA2,CD213A2,CT19,IL-13R,IL13BP Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
SDS-PAGE analysis of Human IL-13 R alpha 2 Protein.
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of Human IL-13 R alpha 2 Protein.
Loaded Human IL-13, Fc Tag (Cat. No. orb612113) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. orb612134) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC | |
63.4 kDa (predicted). Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result. |
98.00% | |
39.5 kDa (predicted). Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result. |
98.00% | |
63.7 kDa (predicted). Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human IL-13 R alpha 2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Asp27-Leu342 ) of Human IL-13 R alpha 2 fused with hFc His Tag tag at the C-terminal. |