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Catalog Number | orb612135 |
---|---|
Category | Proteins |
Description | Human IL-13 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 14.2 kDa |
Target | IL-13 |
Source | Human IL-13, His Tag (orb612135) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Biotinylated Human IL-13 R alpha 1 Protein, His, captured on Biotin CAP-Series S Sensor Chip can bind Human IL-13 Protein, His Tag, premium grade with an affinity constant of 34.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Gly 21 - Asn 132 |
Endotoxins | 0.1 EU per μg |
NCBI | AAK53823.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600, Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa and 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Biotinylated Human IL-13 R alpha 1 Protein, His, Avitag captured on Biotin CAP-Series S Sensor Chip can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. orb612135) with an affinity constant of 34.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Unconjugated | |
90% | |
39.0 kDa | |
Human IL-13 R alpha 2, His Tag (orb612134) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1). |
Greater than 95% as determined by SDS-PAGE. | |
13.4 kDa | |
Mammalian cell |
> 97% as determined by SDS-PAGE and HPLC. | |
12.3 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
12.5 kDa | |
E.Coli |
Unconjugated | |
90% | |
37.7 kDa | |
Human IL-13 R alpha 1 Protein, His Tag (orb257580) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1). |