Human IL-12B / NKSF2 / p40 Protein

• Catalog Number: orb257709
• Category: Proteins
• Description: Human IL-12B, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 36.6 kDa
• Target: IL-12B
• UniProt ID: P29460
• Source: Human IL-12B, His Tag (orb257709) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Loaded Human IL-12 R beta 1, Fc Tag on Protein A Biosensor, can bind Human IL-12B, His Tag with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Ile 23 - Ser 328
• Endotoxins: 1.0 EU per μg
• NCBI: AAH67499.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL12B,CLMF,CLMF2,NKSF,NKSF2,p40
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-12B protein

Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. orb257709) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).