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Catalog Number | orb257708 |
---|---|
Category | Proteins |
Description | Human IL-12B, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 61.3 kDa |
Target | IL-12B |
UniProt ID | P29460 |
Source | Human IL-12B, Fc Tag (orb257708) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-12 R beta 1, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag with a linear range of 0.039-0.625 μg/mL (QC tested). |
Expression Region | Ile 23 - Ser 328 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH67499 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL12B,CLMF,CLMF2,NKSF,NKSF2,p40 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.3 kDa. The protein migrates as 66-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-12B protein
Immobilized Human IL-12 R beta 1, His Tag (Cat. No. orb570283) at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag (Cat. No. orb257708) with a linear range of 0.039-0.625 μg/mL (QC tested).
Unconjugated | |
95% | |
36.6 kDa | |
Human IL-12B, His Tag (orb257709) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1). |