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Catalog Number | orb257707 |
---|---|
Category | Proteins |
Description | Human IL-12A, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 49.2 kDa |
Target | IL-12A, NKSF1, p35 |
UniProt ID | P29459 |
Source | Human IL-12A, Fc Tag (orb257707) is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Arg 23 - Ser 219 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL12A,CLMF,IL-12A,NFSK,NKSF1,P35 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-12A protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
> 95%, determined by SDS-PAGE | |
This protein contains the p35 subunit of human IL12, termed as IL12A (P29459) (Met 1-Ser 219) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |
> 95% by SDS-PAGE. | |
Recombinant Human IL12A/NKSF1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_002178.2) fused with an Fc, 6��His Tag at the C-terminus. |