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Catalog Number | orb1516422 |
---|---|
Category | Proteins |
Description | Recombinant Human IL-12 Protein |
Tag | C-His-Avi |
Concentration | > 0.5 mg/ml |
Form/Appearance | Lyophilized powder |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 45/40 kDa based on Tris-Bis PAGE result. kDa |
Target | Il12b |
UniProt ID | P29460 |
Protein Sequence | 23-328/328 |
Expression System | 3 |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | Interleukin-12 subunit alpha, IL-12, CLMF p35, CLM Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
57.2 kDa. 75.0 kDa is the observed value of non-reducing gel,and the reducing glue is the size of p40 and p35 subunits, and 57.2 kDa is the theoretical value. | |
Baculovirus |
Unconjugated | |
90% | |
73.3 kDa | |
Human IL-35, Fc Tag (orb1184774) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha) (Accession # Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha)). |
Unconjugated | |
95% | |
83.9 kDa | |
Cynomolgus IL-12 R beta 1 Protein, Fc Tag (orb1743005) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Asp 545 (Accession # G7PWY9-1). |
Unconjugated | |
90% | |
59.0 kDa | |
Human IL-12 R beta 1, His Tag (orb570283) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1). |
Unconjugated | |
90% | |
83.5 kDa | |
Human IL-12 R beta 1, Fc Tag (orb570282) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1). |
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