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Catalog Number | orb257591 |
---|---|
Category | Proteins |
Description | Human IL-1 RII, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1). |
Reactivity | Human |
Tag | C-8×His |
Form/Appearance | Powder |
Purity | 98% |
Conjugation | Unconjugated |
MW | 38.9 kDa |
Target | IL-1 RII |
UniProt ID | P27930 |
Source | Human IL-1 RII, His Tag (orb257591) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-1 beta, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-1 RII, His Tag with a linear range of 1-20 ng/mL (QC tested). |
Expression Region | Phe 14 - Glu 343 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_004624.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD121b,IL1RB,IL1R2,CDw121b Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.9 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-1 RII protein
Immobilized Human IL-1 beta, Fc Tag (Cat. No. orb752327) at 5 μg/mL (100 μL/well) can bind Human IL-1 RII, His Tag (Cat. No. orb257591) with a linear range of 1-20 ng/mL (QC tested).
Unconjugated | |
95% | |
63.9 kDa | |
Human IL-1 RII, Fc Tag (orb257592) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1). |