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Catalog Number | orb347289 |
---|---|
Category | Antibodies |
Description | Human IgM (Fc5u) antibody (FITC) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, FC, FLISA, IF |
Reactivity | Human |
Isotype | IgG |
Immunogen | Human IgM Fc5µ fragment |
Concentration | 1.5 mg/mL |
Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes. |
Conjugation | FITC |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti-Human IgM (Fc5u) Antibody fluorescein Read more... |
Note | For research use only |
Application notes | Anti-Human IgM Fc5µ Fluorescein antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Expiration Date | 12 months from date of receipt. |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by trypsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgM Fc5µ and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Trypsin. | |
Human |
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