Human IgG IgA IgM (H&L) antibody (Peroxidase)

• Catalog Number: orb347193
• Category: Antibodies
• Description: Human IgG IgA IgM (H&L) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, IP: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Goat Anti-Human IgG IgA IgM Antibody peroxidase Co
• Note: For research use only
• Application notes: Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Human IgG, IgA, IgM Peroxidase Conjugated Antibody. Lane 1: Human IgG. Lane 2: Human IgA. Lane 3: Human IgM. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: Appropriate banding pattern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms.