Human IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347290
• Category: Antibodies
• Description: Human IgG (H&L) antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Human IgG Antibody peroxidase Conjugat
• Note: For research use only
• Application notes: Anti-Human IgG (H&L) Peroxidase Conjugated Antibody has been tested by ELISA and Dot Blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Rabbit Anti-Human IgG HRP Conjugated Antibody. Human IgG sample loaded 1- 100 ng, 2- 33.3 ng, 3- 11.1 ng, 4- 3.7 ng, 5- 1.2ng. Rabbit Anti-Human IgG Peroxidase Conjugated Antibody 1:1000 for 60 mins at RT. Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 1 hr at RT.

ELISA results of Rabbit anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG protein. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n orb346219). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.