Human IgG (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb347345
• Category: Antibodies
• Description: Human IgG (H&L) antibody (FITC)
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken Serum, Human IgG and Human Serum.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: chicken anti-Human IgG fluorescein conjugated Anti
• Note: For research use only
• Application notes: Anti-Human IgG (H&L) Fluorescein has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Chicken Anti-HUMAN IgG Fluorescein Conjugated Antibody. Lane 1: 100 ng. Lane 2: 33.3 ng. Lane 3: 11.1 ng. Lane 4: 3.7 ng. Lane 5: 1.23 ng. Secondary Antibody: Ch-a-Hu IgG FITC Conj 1 µg/ml. Blocking Buffer: BlockOut orb348644 for 30 min at RT.