Human IgG (H&L) antibody (FITC)

• Catalog Number: orb346651
• Category: Antibodies
• Description: Human IgG (H&L) antibody (FITC)
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Concentration: 10 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Mouse Serum, Human IgG and Human Serum.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Mouse anti-Human IgG Fluorescein Conjugated Antibo
• Note: For research use only
• Application notes: Anti-Human IgG (H&L) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Biorbyt primary and DyLight conjugated secondary antibodies were used to detect: Human transferrin; Alpha 1 anti trypsin; and Human IgG in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in blocking buffer (orb348637) and incubated for 2 hrs at RT. Secondary antibodies diluted 1:10000 in blocking buffer and incubated ~ 1hr at 4°C.