Human IgG (H&L) antibody (Alkaline Phosphatase)

• Catalog Number: orb347232
• Category: Antibodies
• Description: Human IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:4,500, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Human IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: goat anti-Human IgG Alkaline Phosphatase Conjugati
• Note: For research use only
• Application notes: Anti-Human IgG (H&L) Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.4 ng Lane 5 - 2.5 ng. Primary antibody: none. Secondary antibody: Alkaline Phosphatase conjugated Goat anti-Human IgG antibody at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

Western Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase Human secondary antibody at 1:1000 o/n at 4°C. Block: orb348637 for 30 min at RT. Reaction visualized using alkaline phosphatase for 30 seconds at RT. Predicted/Observed size: 50 kDa, 25 kDa for Human IgG.