Human IgG F(c) antibody

• Catalog Number: orb347182
• Category: Antibodies
• Description: Human IgG F(c) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG F(c) fragment
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:15,000 - 1:60,000, IHC: 1:1,000 - 1:3,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab) or Mouse Serum Proteins. Specificity was confirmed by ELISA minimal cross reactivity against Mouse IgG.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Anti Human IgG Fc Antibody Pre-Adsorbed, Goat
• Note: For research use only
• Application notes: Anti-Human IgG F(c) antibody has been tested by dot blot and ELISA. This antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Goat anti-Human IgG F(c) antibody (min x Mouse serum proteins) tested against purified Human IgG F(c) (p/n orb346220). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) as well as Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (p/n orb347032) and TMB substrate p/n orb348651.