Human IgG Fc Antibody

• Catalog Number: orb346220
• Category: Proteins
• Description: Human IgG Fc Antibody
• Tested applications: DOT, ELISA, SDS-PAGE, WB
• Isotype: IgG F(c)
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5000 - 1:50,000, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain.
• Conjugation: Unconjugated
• Source: Human
• Biological Origin: Human
• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Human Immunoglobulin G F(c) Fragment, IgG Fc
• Note: For research use only
• Application notes: HUMAN IgG F(c) Fragment has been tested by SDS-Page and dot blot and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Human IgG Fc (orb346220). Lane 1: Non-reduced Human IgG Fc. Lane 2: Non-reduced Human IgG Whole Molecule (orb346219). Lane 3: Non-reduced Human IgG Fab Fragment (orb346222). Lane 4: Non-reduced Human IgG F(ab')2 (orb346221). Middle Lane: 5 µL OPAL Pre-stained Marker. Lane 5: Reduced Human IgG Fc. Lane 6: Reduced Human IgG Whole Molecule (orb346219). Lane 7: Reduced Human IgG Fab Fragment (orb346222). Lane 8: Reduced Human IgG F(ab')2 (orb346221). Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 50 kDa, reduced at 25 kDa/Non-reduced at 55-60 kDa, reduced at 30-33 kDa.

SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced Human IgG F(c) Fragment. Load: 1 µg. 4-20% Lonza SDS-PAGE; Coomassie Stained.