Human IgG Fc Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347299
• Category: Antibodies
• Description: Human IgG F(c) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Alternative names: Human Fc Antibody, Rb-a-Human F(c), Human IgG F(c)
• Note: For research use only
• Application notes: Anti-Human IgG F(c) Alkaline Phosphatase Conjugated antibody has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Human IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG Fc Load: Lane 1 - 100 ng Lane 2 - 33.33 ng Lane 3 - 11.11 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng Primary antibody: n/a Secondary antibody: Human IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.