Human IgG F(ab')2 Biotin Antibody

• Catalog Number: orb346254
• Category: Proteins
• Description: Human IgG F(ab')2 Biotin Antibody
• Tested applications: DOT, ELISA
• Isotype: IgG F(ab')2
• Concentration: 1.0 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(ab’)2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
• Conjugation: Biotin
• Source: Human
• Biological Origin: Human
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Human IgG F(ab')2 Fragment Biotin Conjugate, Human
• Note: For research use only
• Application notes: Human IgG F(ab')2 fragment Biotin antibody has been tested in dot blot and ELISA and are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Human IgG F(ab')2 Fragment Biotin Conjugated. Antigen: Human IgG F(ab')2 Fragment Biotin Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.