Human IgG F(ab')2 Antibody Biotin Conjugated

• Catalog Number: orb347243
• Category: Antibodies
• Description: Human IgG F(ab')2 antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human IgG F(ab')2 fragment
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c).
• Conjugation: Biotin
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Goat Anti Human IgG Fab2 Antibody Biotin Conjugate
• Note: For research use only
• Application notes: Anti-Human IgG F(ab')2 Biotin Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
• Expiration Date: 12 months from date of receipt.

ELISA of purified Goat Anti-Human IgG F(ab')2 Antibody Biotin Conjugated tested against purified Human IgG F(ab')2 Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab')2 (p/n orb346221). The working dilution is 1:108000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated (p/n orb348767) and TMB substrate (p/n orb348651).