Human IgG Fab Antibody

• Catalog Number: orb346222
• Category: Proteins
• Description: Human IgG Fab Antibody
• Tested applications: SDS-PAGE
• Isotype: IgG Fab
• Concentration: 2.1 mg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Human IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Human IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti- Human IgG and anti- Human IgG F(ab’)2. No reaction was observed against anti- Human IgG F(c) or anti-Papain.
• Conjugation: Unconjugated
• Source: Human
• Biological Origin: Human
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Human Immunoglobulin Fab, F(ab), Fragment antigen-
• Note: For research use only
• Application notes: Human IgG Fab Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS-Page of Human Fab. Lane 1: Human Fab - Non-Reduced. Lane 2: Human Fab - Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 50 kDa, 50 kDa for Human Fab. Predicted/Observed size - Reduced: 25 kDa, 25 kDa for Human Fab. Other band(s): None.

SDS-PAGE results of Human IgG Fab Fragment. Lane 1: reduced Human IgG Fab Fragment (5 ug). Lane 2: reduced Human IgG Fab Fragment (1 ug). Lane 3: Opal Prestained Molecular Weight Ladder. Lane 4: non-reduced Human IgG Fab Fragment (1 ug). Lane 5: non-reduced Human IgG Fab Fragment (5 ug). 4-20% Lonza SDS-PAGE; Coomassie Stained.