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Catalog Number | orb216255 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Human IgG Fab |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, IH, WB |
Reactivity | Human |
Immunogen | Human IgG Fab |
Dilution range | ELISA: 1:5000-1:20000, WB: 1:5000-1:20000, IHC-P: 1:500-1:1000 |
Form/Appearance | 1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, 0.05% Sodium Azide |
Conjugation | Unconjugated |
Source | Mouse |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, 0.05% Sodium Azide |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE | |
Unconjugated | |
Human IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Human IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti- Human IgG and anti- Human IgG F(ab’)2. No reaction was observed against anti- Human IgG F(c) or anti-Papain. | |
Human |
ELISA, FC, IF, IH | |
Human | |
Monoclonal | |
APC |
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