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Catalog Number | orb348791 |
---|---|
Category | Proteins |
Description | Human IGFBP-7, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # NP_001544). |
Reactivity | Human |
Tag | N-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 27.0 kDa |
Target | IGFBP-7 |
UniProt ID | Q16270 |
Source | Human IGFBP-7, His Tag (orb348791) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # NP_001544). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human IGFBP-7, His Tagimmobilized on CM5 Chip can bind Human C1q R1, His Tag with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Asp 30 - Leu 282 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001544 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IBP7,IGFBP7 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IGFBP-7 protein
Human IGFBP-7, His Tag (Cat. No. orb348791) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. orb257231) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 27.0 kDa after removal of the signal peptide. The apparent molecular mass of His-IGFBP7 is approximately 35-40 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
52.6 kDa | |
Human IGFBP-7, Fc Tag (orb1146981) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # Q16270-1). |
Human | |
0.94-60ng/mL | |
0.56ng/mL |