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Catalog Number | orb348805 |
---|---|
Category | Proteins |
Description | Human IGFBP-4, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 27.9 kDa |
Target | IGFBP-4 |
UniProt ID | P22692 |
Source | Human IGFBP-4, His Tag (orb348805) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IGFBP-4, His Tag at 2 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 1-16 ng/mL (QC tested). |
Expression Region | Asp 22 - Glu 258 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001543.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IGFBP4,IBP-4 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IGFBP-4 protein
Immobilized Human IGFBP-4, His Tag (Cat. No. orb348805) at 2 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 1-16 ng/mL (QC tested).
Unconjugated | |
90% | |
52.4 kDa | |
Human IGFBP-4 Protein, Fc Tag (orb1818947) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1). |
> 95 %, by SDS-PAGE under reducing conditions | |
25.75 kDa | |
E.coli -derived human recombinant protein |