Human IGFBP-3 Protein, His Tag

• Catalog Number: orb1146966
• Category: Proteins
• Description: Human IGFBP-3, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1).
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 30.6 kDa
• Target: IGFBP-3
• UniProt ID: P17936
• Source: Human IGFBP-3, His Tag (orb1146966) is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Biotinylated Human IGF-II, ,Fc Tag at 2 μg/mL (100 μL/well) on streptavidin precoated (1 μg/well) plate can bind Human IGFBP-3, His Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
• Expression Region: Gly 28 - Lys 291
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000589.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGFBP3,BP-53,IBP3
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human IGF-II, Avitag, Fc Tag at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (1 μg/well) plate can bind Human IGFBP-3, His Tag (Cat. No. orb1146966) with a linear range of 0.01-0.313 μg/mL (QC tested).