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Catalog Number | orb1806488 |
---|---|
Category | Proteins |
Description | Human IGFBP-2 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Gln325 (Accession # P18065). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 33.3 kDa |
Target | IGFBP-2 |
UniProt ID | P18065 |
Source | Human IGFBP-2 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Gln325 (Accession # P18065). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-2 Protein, His Tag with a linear range of 10-156 ng/mL (QC tested). |
Expression Region | Ala 36 - Gln325 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Powder |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.3 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IGF-I, Fc Tag (Cat. No. orb257564) at 5 μg/mL (100 μL/well) can bind Human IGFBP-2 Protein, His Tag (Cat. No. orb1806488) with a linear range of 10-156 ng/mL (QC tested).
98.00% | |
32.5 kDa (predicted). Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result. |
> 95% by SDS-PAGE. | |
Recombinant Human IGFBP-2 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Glu40-Gln328) of human IGFBP-2 (Accession #NP_000588.2) fused with an initial Met at the N-terminus and a 6��His Tag at the C-terminus. |