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Catalog Number | orb1786132 |
---|---|
Category | Proteins |
Description | Human IGFBP-1 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Asn 259 (Accession # P08833). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 27.2 kDa |
Target | IGFBP-1 |
UniProt ID | P08833 |
Source | Human IGFBP-1 Protein, His Tag (orb1786132) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Asn 259 (Accession # P08833). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-1 Protein, His Tag with a linear range of 0.01-0.156 μg/mL (QC tested). |
Expression Region | Ala 26 - Asn 259 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Powder |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.2 kDa. The protein migrates as 32-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IGF-I, Fc Tag (Cat. No. orb257564) at 5 μg/mL (100 μL/well) can bind Human IGFBP-1 Protein, His Tag (Cat. No. orb1786132) with a linear range of 0.01-0.156 μg/mL (QC tested).
CLIA, EIA, LF | |
> 90% as determined by SDS-PAGE. | |
29.7 kDa | |
HEK293 |
> 97% by SDS-PAGE. | |
Recombinant Human IGFBP-1 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala26-Asn259) of human IGFBP-1 (Accession #NP_000587.1) fused with a 6��His Tag at the C-terminus. |