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Catalog Number | orb594726 |
---|---|
Category | Proteins |
Description | Recombinant Human Insulin-like growth factor I(IGF1),partial (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 7.3 kDa |
UniProt ID | P05019 |
Protein Sequence | TLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA |
Protein Length | Partial |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Biological Activity | The ED50 as determined in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells is typically 1-8 ng/mL |
Expression Region | 52-118aa |
Endotoxins | Less than 0.5 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM NaAc-HAC, pH 4.5 |
Alternative names | Insulin-Like Growth Factor I; IGF-I; Mechano Growt Read more... |
Note | For research use only |
Application notes | Partial |
Expiration Date | 6 months from date of receipt. |
ELISA, FA, HPLC, SDS-PAGE, WB | |
Unconjugated | |
> 95% pure by SDS-PAGE and HPLC analyses. | |
9.1 kDa |
Unconjugated | |
95% | |
129.3 kDa | |
Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
> 97% as determined by SDS-PAGE and HPLC. | |
7.4 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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