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Human IGF-II Protein
Catalog Number: orb257566
| Catalog Number | orb257566 |
|---|---|
| Category | Proteins |
| Description | Human IGF-II, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1). |
| Reactivity | Human |
| Tag | N-Fc |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 34.4 kDa |
| Target | IGF-II |
| UniProt ID | P01344 |
| Source | Human IGF-II, Fc Tag (orb257566) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Human IGFBP-3, His Tag at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag with a linear range of 1.2-39 ng/mL (QC tested). |
| Expression Region | Ala 25 - Glu 91 |
| Endotoxins | 1.0 EU per μg |
| NCBI | P01344 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, pH7.5 |
| Alternative names | IGF2,C11orf43,FLJ22066,FLJ44734,IGF-II,PP9974 Read more... |
| Note | For research use only |
| Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 34.4 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IGF-II protein
Immobilized Human IGFBP-3, His Tag (Cat. No. orb1146966) at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag (Cat. No. orb257566) with a linear range of 1.2-39 ng/mL (QC tested).
- Item 1 of 1
Human IGF2 protein (Active) [orb359165]
> 98% as determined by SDS-PAGE and HPLC.
7.5 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human LongIGF-II Protein [orb1471741]
Greater than 95% as determined by reducing SDS-PAGE.
8.91 KDa
Mammalian
50 μg, 10 μg - Item 1 of 1
Human IGF2 Protein, hFc Tag [orb2321387]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IGF2 is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg 
