Human IGF-I R Protein, Fc Tag

• Catalog Number: orb1496214
• Category: Proteins
• Description: The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mR precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 129.3 kDa
• Target: IGF-I R, CD221
• UniProt ID: P08069
• Source: Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human IGF-I R, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, ,Fc Tag with a linear range of 0.01-0.625 μg/mL (QC tested).
• Expression Region: Glu 31 - Asn 932
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC1821
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 129.3 kDa. The protein migrates as 70-75 kDa and 105-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IGF-I R, Fc Tag (Cat. No. orb1496214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, Avitag, Fc Tag with a linear range of 0.01-0.625 μg/mL (QC tested).