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Catalog Number | orb257559 |
---|---|
Category | Proteins |
Description | Human IFN-gamma R1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 18 - Gly 245 (Accession # AAH05333). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 52.4 kDa |
Target | IFN-gamma R1, IFNGR1 |
UniProt ID | P15260 |
Source | Human IFN-gamma R1, Fc Tag (orb257559) is expressed from human 293 cells (HEK293). It contains AA Glu 18 - Gly 245 (Accession # AAH05333). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IFN-gamma, premium grade at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag with a linear range of 1-10 ng/mL (QC tested). |
Expression Region | Glu 18 - Gly 245 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH05333 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IFNGR1,CD119,CDw119,IFNGR,IFN-gamma-R1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.4 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IFN-gamma R1 protein
Immobilized Human IFN-gamma, premium grade (Cat. No. orb257562) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. orb257559) with a linear range of 1-10 ng/mL (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide. The apparent molecular mass of IFNGR1-hFc is approximately 55-100 kDa due to glycosylation. | |
Mammalian |