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Catalog Number | orb257562 |
---|---|
Category | Proteins |
Description | Human IFN-gamma, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # P01579). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 16.8 kDa |
Target | IFN-gamma, IFNG |
UniProt ID | P01579 |
Source | Human IFN-gamma, premium grade (orb257562) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # AAH70256). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IFN-gamma, premium grade at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag with a linear range of 1-10 ng/mL (QC tested). |
Expression Region | Gln 24 - Gln 166 |
Endotoxins | 0.01 EU per μg |
NCBI | AAH70256 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Interferon-gamma ,Interferon-γ Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 16.8 kDa. The protein migrates as 15 kDa, 19 kDa and 24 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IFN gamma protein
Immobilized Human IFN-gamma, premium grade (Cat. No. orb257562) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. orb257559) with a linear range of 1-10 ng/mL (QC tested).
Greater than 95% as determined by SDS-PAGE. | |
16.88 kDa | |
E.coli |
> 98% as determined by SDS-PAGE and HPLC. | |
16.9 kDa | |
E.Coli |
Unconjugated | |
95% | |
26.6 kDa | |
Human IFN-gamma R1, His Tag (orb257557) is expressed from human 293 cells (HEK293). It contains AA Glu 18 - Gly 245 (Accession # AAH05333). |
Unconjugated | |
95% | |
52.4 kDa | |
Human IFN-gamma R1, Fc Tag (orb257559) is expressed from human 293 cells (HEK293). It contains AA Glu 18 - Gly 245 (Accession # AAH05333). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide. The apparent molecular mass of IFNGR1-hFc is approximately 55-100 kDa due to glycosylation. | |
Mammalian |