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Catalog Number | orb867302 |
---|---|
Category | Proteins |
Description | Human IFN-alpha / beta R1 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 49.0 kDa |
Target | IFN-alpha/beta R1 |
UniProt ID | P17181 |
Source | Human IFN-alpha / beta R1 Protein, His Tag (orb867302) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IFNAR1, His Tag on NTA Biosensor, can bind Human IFN-alpha 2b Protein, Fc Tag with an affinity constant of 0.689 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
Expression Region | Lys 28 - Lys 436 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000620.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IFNAR1,IFNAR,CRF2-1,IFN-R-1,IFNAR-1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 49.0 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human IFNAR1, His Tag (Cat. No. orb867302) on NTA Biosensor, can bind Human IFN-alpha 2b Protein, Fc Tag with an affinity constant of 0.689 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Unconjugated | |
90% | |
73.8 kDa | |
Human IFNAR1, Fc Tag (orb257558) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620). |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 74-95 kDa based on Tris-Bis PAGE result. kDa |
> 95% by SDS-PAGE. | |
Recombinant Human IFN-alpha/beta R1 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Lys28-Lys436) of human IFN-alpha/beta R1 (Accession #P17181) fused with a 6��His Tag at the C-terminus. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human IFN-alpha/beta R1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Lys28-Lys436) of Human IFN-alpha/beta R1 fused with a His Tag and Avi tag at theC-terminal. |