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Catalog Number | orb257558 |
---|---|
Category | Proteins |
Description | Human IFNAR1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 73.8 kDa |
Target | IFN-alpha/beta R1 |
UniProt ID | P17181 |
Source | Human IFNAR1, Fc Tag (orb257558) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Lys 28 - Lys 436 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000620 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IFNAR1,IFNAR,CRF2-1,IFN-R-1,IFNAR-1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.8 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IFN-alpha/beta R1 protein
Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
95% | |
49.0 kDa | |
Human IFN-alpha / beta R1 Protein, His Tag (orb867302) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1). |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 74-95 kDa based on Tris-Bis PAGE result. kDa |
> 95% by SDS-PAGE. | |
Recombinant Human IFN-alpha/beta R1 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Lys28-Lys436) of human IFN-alpha/beta R1 (Accession #P17181) fused with a 6��His Tag at the C-terminus. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human IFN-alpha/beta R1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Lys28-Lys436) of Human IFN-alpha/beta R1 fused with a His Tag and Avi tag at theC-terminal. |