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Catalog Number | orb1147003 |
---|---|
Category | Proteins |
Description | Human IFN-alpha 1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 45.8 kDa |
Target | IFN-alpha 1 |
UniProt ID | P01562 |
Source | Human IFN-alpha 1, Fc Tag (orb1147003) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IFN-alpha / beta R1, His Tag on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
Expression Region | Cys 24 - Glu 189 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_076918.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 |
Alternative names | IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human IFN-alpha / beta R1, His Tag (Cat. No. orb867302) on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. orb1147003) with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 73 kDa after removal of the signal peptide. The apparent molecular mass of IFNAR1-mFc is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
73.8 kDa | |
Human IFNAR1, Fc Tag (orb257558) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of IFNA1-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |