Human ICOS Protein, mFc-His tag

• Catalog Number: orb689387
• Category: Proteins
• Description: Recombinant human ICOS protein with C-terminal mouse Fc and 6×His tag
• Reactivity: Human
• Tag: C-Mouse Fc and 6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 41.1 kDa after removal of the signal peptide.
• Target: ICOS
• UniProt ID: Q9Y6W8
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: ICOS;CD278;AILIM;Inducible T-cell costimulator
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CTLA-4 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein orb689457 can bind Human CTLA4, mFc-His tagged protein (orb689378) in a linear range of 0.048-2.094 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein orb689458 can bind Human CTLA4, mFc-His tagged protein (orb689378) in a linear range of 0.048-2.694 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CTLA-4, mFc-His tagged protein (orb689378) can bind Anti-CTLA-4 Neutralizing antibody orb689327 in a linear range of 0.13-16.0 ng/ml.

Flow cytometry analysis with 1 μg/mL Human CTLA4 Protein, mFc-His tag (orb689378) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).