Human hs-CRP ELISA Kit

• Catalog Number: orb782328
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein(CRP).
• Tested applications: ELISA
• Reactivity: Human
• Range: 1.57-100 ng/mL
• Concentration: 100 ng/mL
• Sensitivity: 0.5 ng/mL
• Protein Length: 3.5h
• Alternative names: C-RP ELISA Kit, PTX1 ELISA Kit, Pentraxin-Related
• Note: For research use only
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.