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Catalog Number | orb257546 |
---|---|
Category | Proteins |
Description | Human HMGB1, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P09429-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 26.8 kDa |
Target | HMGB1 |
UniProt ID | P09429 |
Protein Sequence | NP_002119.1 |
Source | Human HMGB1, His Tag (orb257546) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P09429-1). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Met 1 - Glu 215 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_002119.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | HMGB1,HMG1,HMG3,SBP-1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.8 kDa. The protein migrates as 32 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human HMGB1 protein
Greater than 85% as determined by SDS-PAGE. | |
49.1 kDa | |
Mammalian cell |
Human | |
31.25-2000 pg/mL | |
13.7 pg/mL |
SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
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