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Catalog Number | orb1516433 |
---|---|
Category | Proteins |
Description | Recombinant Human HMGB1 Protein |
Tag | C-His |
Concentration | > 0.5 mg/ml |
Form/Appearance | Lyophilized powder |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. kDa |
Target | HMGB1 |
UniProt ID | P09429 |
Protein Sequence | 1-215/215 |
Expression System | 3 |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | High mobility group protein B1, Amphoterin, High m Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
49.1 kDa | |
Mammalian cell |
Human | |
31.25-2000 pg/mL | |
13.7 pg/mL |
SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
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