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Human GUCY2C protein
Catalog Number: orb605223
| Catalog Number | orb605223 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Heat-stable enterotoxin receptor(GUCY2C),partial |
| Tag | N-terminal 10xHis-tagged |
| Form/Appearance | Lyophilized powder |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| MW | 49.5 kDa |
| UniProt ID | P25092 |
| Protein Sequence | SQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ |
| Protein Length | Extracellular Domain |
| Source | Mammalian cell |
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC50 is 11.89-24.13 ng/mL. |
| Expression Region | 24-430aa |
| Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Alternative names | (STA receptor)(hSTAR)(Guanylyl cyclase C)(GC-C)(In Read more... |
| Note | For research use only |
| Application notes | Extracellular Domain |
| Expiration Date | 6 months from date of receipt. |
Human GUCY2C Protein, His Tag [orb1173928]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46.8 kDa after removal of the signal peptide. The apparent molecular mass of GUCY2C-His is approximately 55-130 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgHuman GUCY2C protein [orb594987]
Greater than 85% as determined by SDS-PAGE.
48.5 kDa
Baculovirus
20 μg, 100 μg, 1 mgRecombinant Human Guanylyl Cyclase C/GUCY2C Protein, Avi His Tag [orb1550517]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
KMP1972, Recombinant Human Guanylyl Cyclase C/GUCY2C Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser24-Gln430) of Human Guanylyl Cyclase C/GUCY2C fused with His Tag and Avi tag at the C-terminal.
100 μg, 500 μgBiotinylated Mouse GUCY2C / Guanylyl cyclase C Protein [orb1496317]
Biotin
90%
50.7 kDa
Biotinylated Mouse GUCY2C, His, (orb1496317) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Met 433 (Accession # Q3UWA6-1).
25 μg, 200 μgHuman GUCY2C Protein [orb1516447]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result. kDa
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