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Catalog Number | orb1516447 |
---|---|
Category | Proteins |
Description | Recombinant Human GUCY2C Protein |
Tag | C-His-Avi |
Concentration | > 0.5 mg/ml |
Form/Appearance | Lyophilized powder |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result. kDa |
Target | GUCY2C |
UniProt ID | P25092 |
Protein Sequence | 24-430/1073 |
Expression System | 3 |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | Heat-stable enterotoxin receptor, STA receptor, hS Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46.8 kDa after removal of the signal peptide. The apparent molecular mass of GUCY2C-His is approximately 55-130 kDa due to glycosylation. | |
Mammalian |
Greater than 95% as determined by SDS-PAGE. | |
49.5 kDa | |
Mammalian cell |
Greater than 85% as determined by SDS-PAGE. | |
48.5 kDa | |
Baculovirus |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP1972, Recombinant Human Guanylyl Cyclase C/GUCY2C Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser24-Gln430) of Human Guanylyl Cyclase C/GUCY2C fused with His Tag and Avi tag at the C-terminal. |
Biotin | |
90% | |
50.7 kDa | |
Biotinylated Mouse GUCY2C, His, (orb1496317) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Met 433 (Accession # Q3UWA6-1). |
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