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Catalog Number | orb257505 |
---|---|
Category | Proteins |
Description | Human GHR, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 29.6 kDa |
Target | Growth Hormone R |
UniProt ID | P10912 |
Source | Human GHR, His Tag (orb257505) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human GH, premium grade at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag with a linear range of 0.078-3 ng/mL (QC tested). |
Expression Region | Ala 27 - Tyr 264 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000154.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | GHR,GHBP,GH receptor Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Nelson, Justin T. et al. Mechanism of immobilized protein A binding to immunoglobulin G on nanosensor array surfaces Anal Chem, 87, 8186-8193 (2015)
SDS-PAGE analysis of Human Growth Hormone R protein
Immobilized Human GH, premium grade at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag (Cat. No. orb257505) with a linear range of 0.078-3 ng/mL (QC tested).
Unconjugated | |
95% | |
54.3 kDa | |
Human GHR, Fc Tag (orb257506) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912). |
Unconjugated | |
90% | |
54.7 kDa | |
Rhesus macaque GHR, Fc Tag (orb545729) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Tyr 264 (Accession # P79194-1). |
Unconjugated | |
90% | |
29.2 kDa | |
Canine Growth Hormone R, His Tag (orb1818956) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Gln 264 (Accession # Q9TU69-1). |
Unconjugated | |
90% | |
30.2 kDa | |
Rat Growth Hormone R, His Tag (orb1496174) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 265 (Accession # P16310-1). |
Unconjugated | |
90% | |
30.2 kDa | |
Cynomolgus Growth Hormone R, His Tag (orb1496205) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Tyr 264 (Accession # EHH62357.1). |