Human Growth Hormone protein

• Catalog Number: orb80030
• Category: Proteins
• Description: GH Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22260.01 Dalton. Growth Hormone is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: GH
• Solubility (25°C): It is recommended to reconstitute the lyophilized HGH in sterile H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile.
• Biological Origin: E.coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of ＞ 1.0 × 10⁷ IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The GH (1mg/ml) was lyophilized after extensive dialyses against 8mg mannitol and 0.34mg sodium phosphate buffer (0.02 mg sodium phosphate monobasic 0.32mg sodium phosphate dibasic).
• Alternative names: GH1 protein, GH protein, GHN protein, GH-N protein
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.72 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of GH as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Growth Hormone protein