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Catalog Number | orb1292297 |
---|---|
Category | Proteins |
Description | Recombinant Human GMFB protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 37.4 KDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
UniProt ID | P60983 |
Source | E.Coli |
Expression System | 1-142 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
Alternative names | Glia maturation factor beta, glia maturation facto Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 97% pure by SDS-PAGE and HPLC analyses. | |
16.4 kDa |
SDS-PAGE | |
Unconjugated | |
> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
≥90% as determined by SDS-PAGE | |
This protein contains the human GMFB(Met1-His142) was fused with the C-terminal His Tag and expressed in E. coli. |
Approximately 16.4 KDa, a single non-glycosylated polypeptide chain containing 141 amino acid residues. | |
Escherichia coli. |
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