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Catalog Number | orb80031 |
---|---|
Category | Proteins |
Description | Granulocyte MCSF Human Recombinant produced in E.coli is a single non-glycosylated polypeptide chain approximately 14.6 kDa, containing 128 amino acids. GMCSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GMCSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GMCSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GMCSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GMCSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GMCSF is species specific and human GMCSF has no biological effects on mouse cells. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. |
Conjugation | Unconjugated |
Target | GM CSF |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is 0.1 ng/ml, corresponding to Specific Activity of 11,100,000 IU/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4 (with an error margin of +/-0.1). |
Alternative names | CSF-2 protein, MGI-1GM protein, GM-CSF protein, Pl Read more... |
Note | For research use only |
Application notes | < strong>Experiment Notes: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of GM-CSF as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human GMCSF protein
Greater than 95% as determined by SDS-PAGE. | |
14.6 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSF-hFc is approximately 35-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
P. pastoris |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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