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Catalog Number | orb545734 |
---|---|
Category | Proteins |
Description | Human GM-CSF R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 36.4 kDa |
Target | GM-CSF R alpha |
UniProt ID | P15509 |
Source | Human GM-CSF R alpha, His Tag (orb545734) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, premium grade with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested). |
Expression Region | Glu 23 - Gly 320 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_006131.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alp Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human GM-CSF R alpha, His Tag (Cat. No. orb545734) captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested).
Unconjugated | |
95% | |
60.9 kDa | |
Human GM-CSF R alpha, Fc Tag (orb545735) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 60.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSFR-hFc is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. kDa |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. kDa |
Greater than 95% as determined by SDS-PAGE. | |
35.5 kDa | |
Mammalian cell |