You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Human GM-CSF Protein
Catalog Number: orb257510
| Catalog Number | orb257510 |
|---|---|
| Category | Proteins |
| Description | Human GM-CSF, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2). |
| Reactivity | Human |
| Tag | Native |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 14.5 kDa |
| Target | GM-CSF |
| UniProt ID | P04141 |
| Source | Human GM-CSF, premium grade (orb257510) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Loaded Human GM-CSF R alpha, Fc Tag on Protein A Biosensor, can bind Human GM-CSF, premium grade with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Ala 18 - Glu 144 |
| Endotoxins | 0.1 EU per μg |
| NCBI | NP_000749.2 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | GM-CSF,CSF2,MGC131935 Read more... |
| Note | For research use only |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 14.5 kDa. The protein migrates as 25 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human GM-CSF protein
Loaded Human GM-CSF R alpha, Fc Tag (Cat. No. orb545735) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Item 1 of 1
Human CSF2 protein [orb594712]
Greater than 95% as determined by SDS-PAGE.
14.6 kDa
E.coli
1 mg, 500 μg, 10 μg, 50 μg - Item 1 of 1
Human CSF2 protein (Active) [orb359028]
> 98% as determined by SDS-PAGE and HPLC.
14.5 kDa
E.Coli
5 μg, 100 μg, 500 μg - Item 1 of 1
Human GM-CSF R alpha Protein [orb545735]
Unconjugated
95%
60.9 kDa
Human GM-CSF R alpha, Fc Tag (orb545735) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1).
100 μg, 1 mg - Item 1 of 1
Human GM-CSF R alpha Protein [orb545734]
Unconjugated
95%
36.4 kDa
Human GM-CSF R alpha, His Tag (orb545734) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1).
1 mg, 100 μg - Item 1 of 2
Human GM-CSFR Protein, hFc Tag [orb1173736]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 60.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSFR-hFc is approximately 70-130 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg
