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Catalog Number | orb1516445 |
---|---|
Category | Proteins |
Description | Recombinant Human GFRAL Protein |
Tag | C-His-Avi |
Concentration | > 0.5 mg/ml |
Form/Appearance | Lyophilized powder |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result. kDa |
Target | GFRAL |
UniProt ID | Q6UXV0 |
Protein Sequence | 19-351/394 |
Expression System | 3 |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | GDNF family receptor alpha-like, Gfral, UNQ9356/PR Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
64.2 kDa | |
Human GFR alpha-like, Fc Tag (orb1146970) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Glu 351 (Accession # Q6UXV0-1). |
Greater than 90% as determined by SDS-PAGE. | |
53.8 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
42.8 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 38.6 kDa after removal of the signal peptide. The apparent molecular mass of GFRAL-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95%by Tris-Bis PAGE, > 95%by HPLC | |
KMP1836, Recombinant Human GFRAL Protein is produced by mammalian expression system. The target protein is expressed with sequence (Ser19-Glu351) of human GFRAL (Accession #) fused with a His Tag and Avi Tag at the C-terminal. |
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