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Human GDNF Protein
Catalog Number: orb1675340
| Catalog Number | orb1675340 |
|---|---|
| Category | Proteins |
| Description | Human GDNF Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P39905-1). |
| Reactivity | Human |
| Tag | Native |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 15.1 kDa |
| Target | GDNF, ATF, hGDNF |
| UniProt ID | P39905 |
| Source | Human GDNF Protein, premium grade (orb1675340) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P39905-1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Human GDNF Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag with a linear range of 0.2-4 ng/mL (QC tested). |
| Expression Region | Ser 78 - Ile 211 |
| Endotoxins | 0.1 EU per μg |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Note | For research use only |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 15.1 kDa. The protein migrates as 15-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Immobilized Human GDNF Protein, premium grade (Cat. No. orb1675340) at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag (Cat. No. orb257499) with a linear range of 0.2-4 ng/mL (QC tested).
- Item 1 of 3
Recombinant human GFRAL protein, C-His-Avi (HEK293) [orb1516445]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
50 μg - Item 1 of 1
Human C6orf144 protein [orb358708]
Greater than 90% as determined by SDS-PAGE.
53.8 kDa
E.coli
20 μg, 1 mg, 100 μg - Item 1 of 1
- Item 1 of 1
Human GDNF Protein, hFc Tag [orb1173742]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 41.2 kDa after removal of the signal peptide. The apparent molecular mass of GDNF-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg 
