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Catalog Number | orb624215 |
---|---|
Category | Proteins |
Description | Human GDF-15, His Tag is expressed from E. coli cells. It contains AA Ala 197 - Ile 308 (Accession # Q99988-1). |
Reactivity | Human |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 14.2 kDa |
Target | GDF-15, MIC-1 |
UniProt ID | Q99988 |
Source | Human GDF-15, His Tag (orb624215) is expressed from E. coli cells. It contains AA Ala 197 - Ile 308 (Accession # Q99988-1). |
Expression System | E. coli |
Biological Origin | Human |
Biological Activity | Immobilized Human GDF-15, His Tag at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag with a linear range of 0.4-25 ng/mL (QC tested). |
Expression Region | Ala 197 - Ile 308 |
Endotoxins | 0.2 EU per μg |
NCBI | NP_004855.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 30% ACN, 0.085% TFA |
Alternative names | GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.2 kDa. the protein migrates as 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
Immobilized Human GDF-15, His Tag (Cat. No. orb624215) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag (Cat. No. orb1146970) with a linear range of 0.4-25 ng/mL (QC tested).
98.00% | |
15.19 kDa (predicted) same as Tris-Bis PAGE result. |
92.50% | |
13.7 kDa (predicted); 18.2 kDa (reducing condition, due to glycosylation) |